bbk 000000

UDC 543.632.495

Medvedeva T. M., Kolokolova N. N., Skriabin M. E.

Investigating iron bacteria in catalytic biofilm in underground waters at the Velizhany water treatment facilities of Tumen


Underground water used for drinking water supply of Tumen is characterized by high concentrations of iron and manganese that 1.3–10.59 times exceed the maximum permissible level. Iron and manganese can be efficiently removed from natural water with the help of microbiological oxidation and sorption processes. The studies aimed at analyzing the underground source for the presence of iron bacteria group and determining the rate of catalytical biofilm formation. Water analysis for the presence of iron bacteria was carried out in sand filters of the Velizhany water treatment facilities by microscopic method with the use of iron cytochrome coloring with potassium ferrocyanide on exposed specimen slides. The slides were placed on styrofoam floats installed in the filters of the water treatment facilities. The results of the studies showed that the water from the underground source contained iron bacteria – they were found in catalytic biofilm. It was stated that the rate of biofilm generation with the participation of iron bacteria in sand filters of the Velizhany water treatment facilities at the media level was in average twice as high compared to the level of surface of the water being treated. Mass growth of iron bacteria on sand media proceeded for 8–12 hours; in the water being treated – for 22–24 hours. It might be caused by biological oxidation of iron and manganese at the media level. The studies carried out confirm the possible use of iron bacteria of catalytic biofilm in biological removal of iron and manganese from underground water at the Velizhany water treatment facilities.

Key words

, , , , , , ,

The further text is accessible on a paid subscription.
For authorisation enter the login/password.
Or subscribe


  1. Troshkova E. A., Moiseev A. V. [Industrial-scale tests of an injection aeration system at the Velizhany water de-ironing plant in Tumen]. Vodosnabzhenie i Sanitarnaia Tekhnika, 2012, no. 5, pp. 30–34. (In Russian).
  2. http://www.vodokanal.info/about/today/velijans/ (accessed April 21, 2014).
  3. Dubinina G. A., Grabovich M. Iu., Churikova V. V., Eprintsev A. T., Churikov S. N. [Investiga­ting microbiological transformation of Mn and Fe in surface and underground water of water intake areas]. Vodnye Resursy, 1999, v. 26, no. 4, pp. 484–488. (In Russian).
  4. Bukreeva V. Iu., Trubitsyn I. V., Dubinina G. A., Grabovich M. Iu., Eprintsev A. T. [Biological activity of iron bacteria associates in the process of laboratory modeling of sand filters depending on the ambient conditions]. Vestnik VGU, Biology Series, 2011, no. 1, pp. 75–79. (In Russian).
  5. Grabovich M. Iu., Dubinina G. A., Bukreeva V. Iu., Eprintsev A. T. Formirovanie mikrobiologiche­skogo sostava peschanykh fil’trov vodopod’emnykh stantsii [Formation of microbiological composition of sand filters at lift stations. Organization and regulation of physiological and biochemical processes. Voronezh, VGU, 2008, issue 10, pp. 298–303].
  6. Bukreeva V. Iu., Grabovich M. Iu., Eprintsev A. T., Dubinina G. A. [Sorption of colloid compounds of iron and manganese oxides on sand media of lift station treatment facilities]. Sorbtsionnye i Khromatograficheskie Protsessy, 2009, v. 9, issue 4, pp. 506–514. (In Russian).
  7. Mencha M. N. [Underground water iron bacteria in de-ironing processes]. Vestnik Brestskogo Gosudarstvennogo Tekhnicheskogo Universiteta, Water utilities construction and power engineering Series, 2006, no. 2 (38), pp. 49–53. (In Russian).
  8. Zenova G. M., Stepanov A. L., Likhacheva A. A., Manucharova N. A. Praktikum po biologii pochv [Practical course on soil biology. Moscow, MSU Press, 2002, 120 p.].
  9. Netrusov A. I., Egorova M. A., Zakharchuk L. M., Kolotilova N. N., et al. Praktikum po mikrobiologii [Practical course on microbiology, Under the editorship of A. I. Netrusov. Moscow, Academiia Publ., 2005, 604 p.].

vstmag engfree 200x100 2

mvkniipr ru

Российская ассоциация водоснабжения и водоотведения

Конференция итог

ecw20 200 300